Cx32R220stop
(Plasmid #78151)

• Purpose: Mammalian Expression of the first 219 amino acids of Connexin 32 and EGFP from a bicistronic mRNA
• Depositing Lab: Mario Bortolozzi
• Publication: Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496. doi: 10.1093/hmg/ddx386.

Backbone

• Vector backbone: pIRES2-EGFP
• Backbone size w/o insert (bp): 5308
• Total vector size (bp): 5950
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Connexin 32
• Alt name: GJB1
• Species: H. sapiens (human)
• Mutation: Only contains first 219 residues
• Entrez Gene: GJB1 (a.k.a. CMTX, CMTX1, CX32)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: IRES-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry