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Addgene

pGEX6p-PBX1
(Plasmid #78145)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78145 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6p-2xrbs
  • Modifications to backbone
    two ribosome binding sites present
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PBX1
  • Alt name
    pre-B-cell leukemia homeobox 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    PBX1 (a.k.a. CAKUHED)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX6p-PBX1 was a gift from Francesco Blasi (Addgene plasmid # 78145 ; http://n2t.net/addgene:78145 ; RRID:Addgene_78145)

  • For your References section:

    Purification and characterization of a DNA-binding recombinant PREP1:PBX1 complex. Mathiasen L, Bruckmann C, Pasqualato S, Blasi F. PLoS One. 2015 Apr 9;10(4):e0125789. doi: 10.1371/journal.pone.0125789. eCollection 2015. PONE-D-14-50577 [pii] PubMed 25856340
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