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Addgene

MBD1v1 pcDNA3.1
(Plasmid #78140)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78140 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MBD1
  • Species
    H. sapiens (human)
  • Mutation
    v1 isoform
  • Entrez Gene
    MBD1 (a.k.a. CXXC3, PCM1, RFT)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MBD1v1 pcDNA3.1 was a gift from Heidi Schwarzenbach (Addgene plasmid # 78140 ; http://n2t.net/addgene:78140 ; RRID:Addgene_78140)

  • For your References section:

    Methyl-CpG binding domain proteins and their involvement in the regulation of the MAGE-A1, MAGE-A2, MAGE-A3, and MAGE-A12 gene promoters. Wischnewski F, Friese O, Pantel K, Schwarzenbach H. Mol Cancer Res. 2007 Jul;5(7):749-59. 10.1158/1541-7786.MCR-06-0364 PubMed 17634428
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