Skip to main content
Addgene

MAGE-A12Pro pGL4basic
(Plasmid #78139)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGL4basic
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAGE-A12
  • Species
    H. sapiens (human)
  • Mutation
    contains -425/+115 of promoter
  • Entrez Gene
    MAGEA12 (a.k.a. CT1.12, MAGE12)
  • Promoter MAGE-A12
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNRev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAGE-A12Pro pGL4basic was a gift from Heidi Schwarzenbach (Addgene plasmid # 78139 ; http://n2t.net/addgene:78139 ; RRID:Addgene_78139)

  • For your References section:

    Methyl-CpG binding domain proteins and their involvement in the regulation of the MAGE-A1, MAGE-A2, MAGE-A3, and MAGE-A12 gene promoters. Wischnewski F, Friese O, Pantel K, Schwarzenbach H. Mol Cancer Res. 2007 Jul;5(7):749-59. 10.1158/1541-7786.MCR-06-0364 PubMed 17634428
Related items:
Commonly requested with: