pcDNA3_erGAP2
(Plasmid #78120)

• Purpose: Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum
• Depositing Labs: Teresa Alonso, Javier García-Sancho
• Publication: pcDNA3_erGAP2 (unpublished)

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5397
• Total vector size (bp): 6818
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: erGAP2
• Alt name: Endoplasmic reticulum targeted GAP2
• Species: Synthetic
• Insert Size (bp): 1421
• Mutation: D24N/D119A in aequorin moiety
• Promoter: CMV
• Tags / Fusion Proteins:
  • calreticulin signal peptide (N terminal on backbone)
  • KDEL (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry