pcDNA3.1-GNPTAB
(Plasmid
#78108)
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PurposeMammalian expression of human GNPTAB with C terminal myc tag
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78108 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGNPTAB
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SpeciesH. sapiens (human)
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Entrez GeneGNPTAB (a.k.a. GNPTA, ICD)
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-GNPTAB was a gift from Thomas Braulke (Addgene plasmid # 78108 ; http://n2t.net/addgene:78108 ; RRID:Addgene_78108) -
For your References section:
Transport of the GlcNAc-1-phosphotransferase alpha/beta-subunit precursor protein to the Golgi apparatus requires a combinatorial sorting motif. Franke M, Braulke T, Storch S. J Biol Chem. 2013 Jan 11;288(2):1238-49. doi: 10.1074/jbc.M112.407676. Epub 2012 Nov 28. 10.1074/jbc.M112.407676 PubMed 23192343