pcDNA3.1-GNPTAB
(Plasmid
#78107)
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PurposeMammalian expression of human GNPTAB, the alpha/beta subunit precursor miniconstruct that allows its efficient expression, spans the membrane twice, and lacks amino acids 431 to 819.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78107 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGNPTAB
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SpeciesH. sapiens (human)
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Entrez GeneGNPTAB (a.k.a. GNPTA, ICD)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-GNPTAB was a gift from Thomas Braulke (Addgene plasmid # 78107 ; http://n2t.net/addgene:78107 ; RRID:Addgene_78107) -
For your References section:
A key enzyme in the biogenesis of lysosomes is a protease that regulates cholesterol metabolism. Marschner K, Kollmann K, Schweizer M, Braulke T, Pohl S. Science. 2011 Jul 1;333(6038):87-90. doi: 10.1126/science.1205677. 10.1126/science.1205677 PubMed 21719679