pcDNA3.1-mGnptg
(Plasmid
#78106)
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PurposeMammalian expression of mouse Gnptg with C-terminal His tag
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78106 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1/Hygro(+)
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGnptg
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SpeciesM. musculus (mouse)
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GenBank IDNM_172529.3
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Entrez GeneGnptg (a.k.a. 6430527N14Rik, A830081F19, AU067667, AU067744, Mdcp1, Tce7)
- Promoter CMV
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-mGnptg was a gift from Thomas Braulke (Addgene plasmid # 78106 ; http://n2t.net/addgene:78106 ; RRID:Addgene_78106) -
For your References section:
Post-translational modifications of the gamma-subunit affect intracellular trafficking and complex assembly of GlcNAc-1-phosphotransferase. Encarnacao M, Kollmann K, Trusch M, Braulke T, Pohl S. J Biol Chem. 2011 Feb 18;286(7):5311-8. doi: 10.1074/jbc.M110.202382. Epub 2010 Dec 20. 10.1074/jbc.M110.202382 PubMed 21173149