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Purpose(Empty Backbone) Plant expression of Cas9 and gRNA empty backbone; Basta resistance
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78097 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepB2GW7
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Modifications to backboneAt U6 promoter driven sgRNA cassette, human codon-optimized SpCas9 gene containing a nuclear localization signal (NLS) and a HA tag at the Cterminus of SpCas9
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Vector typePlant Expression, CRISPR
- Promoter U6
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Selectable markersBasta
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Tag
/ Fusion Protein
- NLS-HA (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
protocol for cloning gRNA can be found in associated article.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAtC was a gift from Jin-Soo Kim (Addgene plasmid # 78097 ; http://n2t.net/addgene:78097 ; RRID:Addgene_78097) -
For your References section:
A simple, flexible and high-throughput cloning system for plant genome editing via CRISPR-Cas system. Kim H, Kim ST, Ryu J, Choi MK, Kweon J, Kang BC, Ahn HM, Bae S, Kim JS, Kim SG. J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474. 10.1111/jipb.12474 PubMed 26946469
