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Addgene

pXJ40 GADD34 (Delta Pest1)
(Plasmid #78096)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78096 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXJ40
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GADD34
  • Alt name
    protein phosphatase 1 regulatory subunit 15A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1866
  • Mutation
    deletion of PEST1
  • Entrez Gene
    PPP1R15A (a.k.a. GADD34)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Matt Brush

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS identified R251P, K277E, K430N, and T597A in the GADD34 translation in this plasmid. The depositing lab notes that there are a number of natural variants of this protein, and these amino acid variations likely make no significant difference to the function of the GADD34 protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXJ40 GADD34 (Delta Pest1) was a gift from Shirish Shenolikar (Addgene plasmid # 78096 ; http://n2t.net/addgene:78096 ; RRID:Addgene_78096)

  • For your References section:

    Structural and Functional Analysis of the GADD34:PP1 eIF2alpha Phosphatase. Choy MS, Yusoff P, Lee IC, Newton JC, Goh CW, Page R, Shenolikar S, Peti W. Cell Rep. 2015 Jun 30;11(12):1885-91. doi: 10.1016/j.celrep.2015.05.043. Epub 2015 Jun 18. 10.1016/j.celrep.2015.05.043 PubMed 26095357
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