pXJ40 GADD34 (514-674aa)
(Plasmid #75480)

• Purpose: mammalian expression of GADD34 514-674 aa
• Depositing Lab: Shirish Shenolikar
• Publication: Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. doi: 10.1016/j.celrep.2015.05.043. Epub 2015 Jun 18.

Backbone

• Vector backbone: pXJ40
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GADD34
• Alt name: protein phosphatase 1 regulatory subunit 15A
• Species: H. sapiens (human)
• Insert Size (bp): 1539
• Mutation: expresses aa 514-674
• Entrez Gene: PPP1R15A (a.k.a. GADD34)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Matt Brush

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Our sequencing results with T7 found extra GSKLM amino acids immediately following the FLAG tag and before aa 514 and the start of the GADD34 coding fragment. Further found T597A within the GADD34 translation compared to NP_055145.3. The depositor states that these mutations do not affect plasmid function.