pXJ40 GADD34 Wt (1-674aa)
(Plasmid #75478)

• Purpose: mammalian expression of GADD34
• Depositing Lab: Shirish Shenolikar
• Publication: Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. doi: 10.1016/j.celrep.2015.05.043. Epub 2015 Jun 18.

Backbone

• Vector backbone: pXJ40
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GADD34
• Alt name: protein phosphatase 1 regulatory subunit 15A
• Species: H. sapiens (human)
• Insert Size (bp): 2022
• Entrez Gene: PPP1R15A (a.k.a. GADD34)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Matt Brush

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Our sequencing results with T7 (5'-TAATACGACTCACTATAGGG-3') found extra SGSKL amino acids immediately following the FLAG tag and before the start of the GADD34 coding fragment. The depositor states that this discrepancy does not affect plasmid function.