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Addgene

pJK571
(Plasmid #75379)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75379 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 2710
  • Total vector size (bp) 5962
  • Modifications to backbone
    removed NdeI (vector) - NdeI (insert) segment, deleting 150 bp of vector (including lacZ promoter and M13-forward primer
  • Vector type
    Bacterial Expression ; gene synthesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    formylglycinamidine ribonucleotide synthetase, small subunit
  • Alt name
    purS
  • Alt name
    AZ09_06375
  • Species
    Acetobacter aceti 1023
  • Mutation
    synthetic gene
  • GenBank ID
    KDE20458
  • Promoter n/a

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    hypothetical protein in purSQL operon
  • Alt name
    orfY
  • Alt name
    AZ09_06380
  • Species
    Acetobacter aceti 1023
  • Mutation
    synthetic gene
  • GenBank ID
    KDE20459
  • Promoter n/a
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
  • Alt name
    purQ
  • Alt name
    AZ09_06385
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE20460
  • Promoter n/a

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    formylglycinamidine ribonucleotide synthetase, large subunit
  • Alt name
    purL
  • Alt name
    AZ09_06390
  • Species
    Acetobacter aceti 1023
  • Mutation
    changes glycine-209 to aspartate
  • GenBank ID
    KDE20461
  • Promoter n/a

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer M13_reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK571 was a gift from T. J. Kappock (Addgene plasmid # 75379 ; http://n2t.net/addgene:75379 ; RRID:Addgene_75379)