pJK571
(Plasmid
#75379)
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PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 75379 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
- Backbone size w/o insert (bp) 2710
- Total vector size (bp) 5962
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Modifications to backboneremoved NdeI (vector) - NdeI (insert) segment, deleting 150 bp of vector (including lacZ promoter and M13-forward primer
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Vector typeBacterial Expression ; gene synthesis
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameformylglycinamidine ribonucleotide synthetase, small subunit
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Alt namepurS
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Alt nameAZ09_06375
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SpeciesAcetobacter aceti 1023
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Mutationsynthetic gene
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GenBank IDKDE20458
- Promoter n/a
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namehypothetical protein in purSQL operon
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Alt nameorfY
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Alt nameAZ09_06380
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SpeciesAcetobacter aceti 1023
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Mutationsynthetic gene
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GenBank IDKDE20459
- Promoter n/a
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Tag
/ Fusion Protein
- His6 (N terminal on insert)
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert nameformylglycinamidine ribonucleotide synthetase, glutaminase subunit
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Alt namepurQ
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Alt nameAZ09_06385
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SpeciesAcetobacter aceti 1023
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GenBank IDKDE20460
- Promoter n/a
Cloning Information for Gene/Insert 3
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 4
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Gene/Insert nameformylglycinamidine ribonucleotide synthetase, large subunit
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Alt namepurL
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Alt nameAZ09_06390
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SpeciesAcetobacter aceti 1023
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Mutationchanges glycine-209 to aspartate
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GenBank IDKDE20461
- Promoter n/a
Cloning Information for Gene/Insert 4
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer M13_reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK571 was a gift from T. J. Kappock (Addgene plasmid # 75379 ; http://n2t.net/addgene:75379 ; RRID:Addgene_75379)