CRY2(535, L348F)-mCherry
(Plasmid #75372)

• Purpose: Expresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherry
• Depositing Lab: Chandra Tucker
• Publication: Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063.

Backbone

• Vector backbone: pmCherryN1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRY2 residues 1-535
• Alt name: CRY2
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1495
• Mutation: L348F
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XmaI (not destroyed)
• 5′ sequencing primer: CMV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry