Gal4BD-CRY2(535)
(Plasmid
#75369)
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PurposeYeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 75369 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDBTrp
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCRY2
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)2000
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Entrez GeneCRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
- Promoter ADH1
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Tag
/ Fusion Protein
- Gal4BD (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Gal4BD primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Gal4BD-CRY2(535) was a gift from Chandra Tucker (Addgene plasmid # 75369 ; http://n2t.net/addgene:75369 ; RRID:Addgene_75369) -
For your References section:
Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. Taslimi A, Zoltowski B, Miranda JG, Pathak GP, Hughes RM, Tucker CL. Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063. 10.1038/nchembio.2063 PubMed 27065233
Map uploaded by the depositor.
