CRY2(L348F)-CreN
(Plasmid #75368)

• Purpose: Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase
• Depositing Lab: Chandra Tucker
• Publication: Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063.

Backbone

• Vector backbone: mCherryC1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Modifications to backbone: Contains IRES sequence between mCherry and CRY2-CreN
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRY2
• Species: A. thaliana (mustard weed)
• Mutation: L348F
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
• Promoter: CMV
• Tag / Fusion Protein:
  • Cre recombinase, residues 19-104 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XmaI (not destroyed)
• 5′ sequencing primer: CMV

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry