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Addgene

CIB1-CreC(N1)
(Plasmid #75367)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75367 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mCherryN1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CIB1
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Cre DNA recombinase, residues 106-343 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XmaI (not destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CIB1-CreC(N1) was a gift from Chandra Tucker (Addgene plasmid # 75367 ; http://n2t.net/addgene:75367 ; RRID:Addgene_75367)

  • For your References section:

    Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. Taslimi A, Zoltowski B, Miranda JG, Pathak GP, Hughes RM, Tucker CL. Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063. 10.1038/nchembio.2063 PubMed 27065233
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