CIB81-pmGFP
(Plasmid #75366)

• Purpose: Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end
• Depositing Lab: Chandra Tucker
• Publication: Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063.

Backbone

• Vector backbone: EGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Modifications to backbone: C-term CaaX prenylation motif at end of EGFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CIB1
• Alt name: CIB81
• Species: A. thaliana (mustard weed)
• Mutation: residues 1-81 of CIB1
• Entrez Gene: CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
• Promoter: CMV
• Tag / Fusion Protein:
  • prenylated EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: CMV promoter

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry