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Addgene

pmGFPS
(Plasmid #75360)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75360 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    YDp-H
  • Backbone size w/o insert (bp) 3865
  • Total vector size (bp) 4593
  • Vector type
    integrating vector
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFPmut3
  • Alt name
    mGFP
  • Alt name
    monomeric GFP
  • Species
    Synthetic
  • Insert Size (bp)
    725
  • Mutation
    A206K, S65G, S72A
  • Promoter Lac
  • Tag / Fusion Protein
    • HIS3 (from parental YDp-H) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Sma1 (unknown if destroyed)
  • 5′ sequencing primer CTGCAAGGCGATTAAG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GFPmut3 sequence was obtained from A. Badrinarayanan, Department of Biochemistry, University of Oxford, UK

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmGFPS was a gift from Stefan Hohmann (Addgene plasmid # 75360)
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