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pColdII-SIRPT1-Cucumis sativus
(Plasmid #75357)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75357 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pColdII
  • Backbone manufacturer
    Takara (Japan)
  • Backbone size w/o insert (bp) 4392
  • Total vector size (bp) 8256
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sacsin Cucumis sativus 1-1292
  • Species
    Synthetic; E. coli optimized
  • Insert Size (bp)
    3876
  • Tag / Fusion Protein
    • His-Tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pCold™-F 5'-ACGCCATATCGCCGAAAGG
  • 3′ sequencing primer pCold™-R 5'-GGCAGGGATCTTAGATTCTG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    synthesized and cloned at Biobasic inc.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pColdII-SIRPT1-Cucumis sativus was a gift from Kalle Gehring (Addgene plasmid # 75357 ; http://n2t.net/addgene:75357 ; RRID:Addgene_75357)
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