pColdII-SIRPT1-Latimeria chalumnae
(Plasmid #75356)

• Purpose: Nt His-tag Latimeria chalumnae Sacsin 94-1368
• Depositing Lab: Kalle Gehring
• Publication: Sacsin | SIRPT1 (unpublished)

Backbone

• Vector backbone: pColdII
• Backbone manufacturer: Takara (Japan)
• Backbone size w/o insert (bp): 4392
• Total vector size (bp): 8208
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Sacsin Latimeria chalumnae 94-1368
• Species: Synthetic; E. coli optimized
• Insert Size (bp): 3825
• Tag / Fusion Protein:
  • His-Tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pCold™-F 5'-ACGCCATATCGCCGAAAGG
• 3′ sequencing primer: pCold™-R 5'-GGCAGGGATCTTAGATTCTG

Resource Information

• Supplemental Documents:
  • Map
• A portion of this plasmid was derived from a plasmid made by: synthesized and cloned at Biobasic inc.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry