pME-TagRFPt-NS
(Plasmid
#75343)
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PurposeMultisite gateway entry clone for adding N-terminal fusions of TagRFPt
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 75343 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2551
- Total vector size (bp) 3283
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTagRFPt
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SpeciesSynthetic
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Insert Size (bp)732
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-TagRFPt-NS was a gift from Rob Parton (Addgene plasmid # 75343 ; http://n2t.net/addgene:75343 ; RRID:Addgene_75343) -
For your References section:
A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease. Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ. Zebrafish. 2016 Sep 15. 10.1089/zeb.2016.1321 PubMed 27631880
Map uploaded by the depositor.
