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Addgene

Puro-iNgn3
(Plasmid #75337)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75337 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N/A
  • Total vector size (bp) 7954
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    neurogenin 3
  • Alt name
    Ngn3
  • Alt name
    Neurog3
  • Species
    M. musculus (mouse)
  • GenBank ID
    50674
  • Entrez Gene
    Neurog3 (a.k.a. Atoh5, Math4B, bHLHa7, ngn3)
  • Promoter TRE-tight

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer cagctcaggttctgggagag
  • 3′ sequencing primer gaaatttgtgatgctattgc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Puro-iNgn3 was a gift from Danwei Huangfu (Addgene plasmid # 75337 ; http://n2t.net/addgene:75337 ; RRID:Addgene_75337)

  • For your References section:

    Genome Editing of Lineage Determinants in Human Pluripotent Stem Cells Reveals Mechanisms of Pancreatic Development and Diabetes. Zhu Z, Li QV, Lee K, Rosen BP, Gonzalez F, Soh CL, Huangfu D. Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 10.1016/j.stem.2016.03.015. 10.1016/j.stem.2016.03.015 PubMed 27133796
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