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Addgene

H2BK120C
(Plasmid #75332)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75332 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET3a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4640
  • Total vector size (bp) 5018
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    H2BK120C
  • Alt name
    hist1h2bj
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    300
  • Mutation
    truncated; K120C
  • GenBank ID
  • Entrez Gene
    h2bc11.S (a.k.a. XELAEV_18000725mg, h2B, h2bfr, hist1h2bj, hist1h2bj.L, hist1h2bj.S)
  • Promoter T7
  • Tag / Fusion Protein
    • none

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H2BK120C was a gift from Cynthia Wolberger (Addgene plasmid # 75332 ; http://n2t.net/addgene:75332 ; RRID:Addgene_75332)

  • For your References section:

    Structural basis for histone H2B deubiquitination by the SAGA DUB module. Morgan MT, Haj-Yahya M, Ringel AE, Bandi P, Brik A, Wolberger C. Science. 2016 Feb 12;351(6274):725-8. doi: 10.1126/science.aac5681. 10.1126/science.aac5681 PubMed 26912860
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