His6TEV-UbG76C
(Plasmid #75299)

• Purpose: Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease
• Depositing Lab: Cynthia Wolberger
• Publication: Morgan et al Science. 2016 Feb 12;351(6274):725-8. doi: 10.1126/science.aac5681.

Backbone

• Vector backbone: pET3a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4640
• Total vector size (bp): 4910
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: human Ubiquitin mutant G76 to C
• Species: H. sapiens (human)
• Insert Size (bp): 270
• Mutation: changed Glycine 76 to Cystine
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry