pGEX4T-HEPN
(Plasmid #75296)

• Purpose: Expresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)
• Depositing Lab: Kalle Gehring
• Publication: Kozlov et al J Biol Chem. 2011 Jun 10;286(23):20407-12. doi: 10.1074/jbc.M111.232884. Epub 2011 Apr 20.

Backbone

• Vector backbone: pGEX6P-1
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 4969
• Total vector size (bp): 5374
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Human Sacsin 4441-4579
• Species: H. sapiens (human)
• Insert Size (bp): 414
• Tag / Fusion Protein:
  • GST-Tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: pGEXF
• 3′ sequencing primer: pGEXR

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry