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Addgene

pYFP-RPB1aAmr
(Plasmid #75284)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75284 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEYFP-C1
  • Backbone manufacturer
    BD Biosciences Clontech
  • Backbone size w/o insert (bp) 4731
  • Total vector size (bp) 10646
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    POLR2A
  • Alt name
    RPB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5911
  • Mutation
    N792D alpha-amanitin–resistant
  • GenBank ID
    NM_000937.4
  • Entrez Gene
    POLR2A (a.k.a. NEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1)
  • Promoter CMV
  • Tag / Fusion Protein
    • EYFP (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CMV Forward CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer pX-C1 REVseq TATGTTTCAGGTTCAGGGGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYFP-RPB1aAmr was a gift from Xavier Darzacq & Robert Singer (Addgene plasmid # 75284 ; http://n2t.net/addgene:75284 ; RRID:Addgene_75284)

  • For your References section:

    In vivo dynamics of RNA polymerase II transcription. Darzacq X, Shav-Tal Y, de Turris V, Brody Y, Shenoy SM, Phair RD, Singer RH. Nat Struct Mol Biol. 2007 Sep;14(9):796-806. Epub 2007 Aug 5. 10.1038/nsmb1280 PubMed 17676063
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