pBabe-Kras Wt
(Plasmid #75282)

• Purpose: Expresses human Kras wild-type
• Depositing Lab: Channing Der
• Publication: Mutant Ras Constructs (unpublished)

Backbone

• Vector backbone: pBabe
• Backbone size w/o insert (bp): 5169
• Total vector size (bp): 5906
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: KRAS
• Alt name: K-ras
• Alt name: Kras
• Alt name: Kirsten rat sarcoma viral oncogene homolog
• Species: H. sapiens (human)
• Mutation: None. Wild-Type
• GenBank ID: NC_000012.12
• Entrez Gene: KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBabe 5'
• 3′ sequencing primer: pBabe 3'

Resource Information

• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry