pcDNA3-optoB2-LYY-YFP
(Plasmid
#75271)
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PurposeG-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 75271 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Total vector size (bp) 7372
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameG-protein independent version of optoB2
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Mutationpoint mutations at L72F,Y136G,Y224A
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Tag
/ Fusion Protein
- eYFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-optoB2-LYY-YFP was a gift from Michael Bruchas (Addgene plasmid # 75271 ; http://n2t.net/addgene:75271 ; RRID:Addgene_75271) -
For your References section:
Optodynamic simulation of beta-adrenergic receptor signalling. Siuda ER, McCall JG, Al-Hasani R, Shin G, Il Park S, Schmidt MJ, Anderson SL, Planer WJ, Rogers JA, Bruchas MR. Nat Commun. 2015 Sep 28;6:8480. doi: 10.1038/ncomms9480. 10.1038/ncomms9480 PubMed 26412387
Map uploaded by the depositor.
