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Addgene

pAAV-CRY2-CreN Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 75268 with SpeI-HF and NotI-HF.

We predict the following banding patterns:

SpeI-HF single digest: 6.8kb

NotI-HF single digest: 6.8kb

SpeI-HF / NotI-HF double digest: 4.9kb & 1.9kb

We see the expected result.