PA-Cdc42
(Plasmid #75263)

• Purpose: Light-activated Cdc42
• Depositing Lab: Klaus Hahn
• Publication: Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Backbone

• Vector backbone: pTriEx
• Backbone size w/o insert (bp): 5040
• Total vector size (bp): 6747
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cell Division Cycle 42
• Species: H. sapiens (human)
• Insert Size (bp): 564
• Mutation: F56W
• GenBank ID: NM_001039802.1
• Entrez Gene: CDC42 (a.k.a. CDC42Hs, G25K, TKS)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 6xHIs (N terminal on insert)
  • mVenus (N terminal on insert)
  • LOV2 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: aagtatcgggccctttgtgc
• 3′ sequencing primer: GGCAGCCTGCACCTGAGGTTAATCAC

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry