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Addgene

pQCXIX_sh_HOIP/RNF31_#6
(Plasmid #75252)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75252 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIX (modified)
  • Total vector size (bp) 7735
  • Modifications to backbone
    includes EF1a-EGFP expression cassette, mU6-shRNA expression cassette
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    shRNA against human RNF31
  • Alt name
    HOIP
  • gRNA/shRNA sequence
    GCTGTGCTATGTTCCATAT
  • GenBank ID
    55072 (GENE-ID)
  • Entrez Gene
    RNF31 (a.k.a. HOIP, IMD115, Paul, ZIBRA)
  • Promoter mU6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer CCC TAA CTG TAA AGT AAT TG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Produced ~60 % KD (Western Blot) in Jurkat cells after RV-transduction and sort for GFPpositive cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIX_sh_HOIP/RNF31_#6 was a gift from Ria Baumgrass (Addgene plasmid # 75252 ; http://n2t.net/addgene:75252 ; RRID:Addgene_75252)

  • For your References section:

    Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel H, Radbruch A, Krause E, Baumgrass R. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326. 10.1074/jbc.M116.739326 PubMed 27637333
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