RCAS-sgATG7
(Plasmid #75228)

• Purpose: An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh
• Depositing Lab: Noor Gammoh
• Publication: Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi: 10.15252/embr.201947734. Epub 2019 Aug 26.

Backbone

• Vector backbone: RCAS-Y
• Backbone size w/o insert (bp): 10000
• Total vector size (bp): 10500
• Modifications to backbone: Addition of U6 promoter-driven expression of CRISPR guide RNA against ATG7
• Vector type: Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Autophagy Related 7
• Alt name: ATG7
• gRNA/shRNA sequence: gRNA against ATG7
• Species: M. musculus (mouse)
• Entrez Gene: Atg7 (a.k.a. 1810013K23Rik, Agp7, Apg7l, Atg7l, Gm21553)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CTCTGCTGGTGGCCTCGCGTACCACTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry