Human NCKX6-FlagTag (pcDNA3.1+)
(Plasmid #75217)

• Purpose: Mammalian expression of SLC8B1
• Depositing Lab: Jonathan Lytton
• Publication: Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76. Epub 2003 Nov 18.

Backbone

• Vector backbone: pcDNA3.1+
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SLC8B1
• Alt name: NCKX6/NCLX
• Species: H. sapiens (human)
• Entrez Gene: SLC8B1 (a.k.a. NCKX6, NCLX, SLC24A6)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • Annotated sequence
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

FLAG tag is inserted internal to the protein coding region, as annotated in the annotated sequence file.