Mouse NCKX6 (pcDNA3.1+)
(Plasmid
#75215)
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PurposeMammalian expression of SLC8B1
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 75215 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1+
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSLC8B1
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Alt nameNCKX6/NCLX
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SpeciesM. musculus (mouse)
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Entrez GeneSlc8b1 (a.k.a. AF261233, NCKX6, NCLX, Slc24a6)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mouse NCKX6 (pcDNA3.1+) was a gift from Jonathan Lytton (Addgene plasmid # 75215 ; http://n2t.net/addgene:75215 ; RRID:Addgene_75215) -
For your References section:
Molecular cloning of a sixth member of the K+-dependent Na+/Ca2+ exchanger gene family, NCKX6. Cai X, Lytton J. J Biol Chem. 2004 Feb 13;279(7):5867-76. Epub 2003 Nov 18. 10.1074/jbc.M310908200 PubMed 14625281
