Human NCKX4-AS-short-FlagTag (pcDNA3.1+)
(Plasmid #75212)

• Purpose: Mammalian expression of SLC24A4
• Depositing Lab: Jonathan Lytton
• Publication: Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7. Epub 2002 Oct 11.

Backbone

• Vector backbone: pcDNA3.1+
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SLC24A4
• Alt name: NCKX4-AS-
• Species: H. sapiens (human)
• Mutation: alternatively spliced exon absent
• Entrez Gene: SLC24A4 (a.k.a. AI2A5, NCKX4, SHEP6, SLC24A2)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • Annotated sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

FLAG tag is inserted internal to the protein coding region, as annotated in the annotated sequence file.