Human NCKX3-FlagTag (pcDNA3.1-)
(Plasmid #75206)

• Purpose: Mammalian expression of SLC24A3
• Depositing Lab: Jonathan Lytton
• Publication: Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-72. Epub 2001 Apr 9.

Backbone

• Vector backbone: pcDNA3.1-
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SLC24A3
• Alt name: NCKX3
• Species: H. sapiens (human)
• Entrez Gene: SLC24A3 (a.k.a. NCKX3)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • Annotated sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

FLAG tag is inserted internal to the protein coding region, as annotated in the annotated sequence file.

Note, in addition to the flag tag, this plasmid differs from Plasmid 75205: Human NCKX3 (pcDNA3.1+) in that it includes a long 3' UTR sequence after the NCX3 ORF.