Mouse NCKX3 (pcDNA3.1+)
(Plasmid
#75204)
-
PurposeMammalian expression of SLC24A3
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 75204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3.1+
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSLC24A3
-
Alt nameNCKX3
-
SpeciesM. musculus (mouse)
-
Entrez GeneSlc24a3 (a.k.a. NCKX3)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Mouse NCKX3 (pcDNA3.1+) was a gift from Jonathan Lytton (Addgene plasmid # 75204 ; http://n2t.net/addgene:75204 ; RRID:Addgene_75204) -
For your References section:
Molecular cloning of a third member of the potassium-dependent sodium-calcium exchanger gene family, NCKX3. Kraev A, Quednau BD, Leach S, Li XF, Dong H, Winkfein R, Perizzolo M, Cai X, Yang R, Philipson KD, Lytton J. J Biol Chem. 2001 Jun 22;276(25):23161-72. Epub 2001 Apr 9. 10.1074/jbc.M102314200 PubMed 11294880