Human NCKX2-AS-short (pcDNA3.1+)
(Plasmid
#75200)
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PurposeMammalian expression of SLC24A2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 75200 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1+
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSLC24A2
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Alt nameNCKX2-AS-
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SpeciesH. sapiens (human)
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Mutationalternatively spliced exon absent
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Entrez GeneSLC24A2 (a.k.a. NCKX2)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Human NCKX2-AS-short (pcDNA3.1+) was a gift from Jonathan Lytton (Addgene plasmid # 75200 ; http://n2t.net/addgene:75200 ; RRID:Addgene_75200) -
For your References section:
Exchangers NCKX2, NCKX3, and NCKX4: identification of Thr-551 as a key residue in defining the apparent K(+) affinity of NCKX2. Visser F, Valsecchi V, Annunziato L, Lytton J. J Biol Chem. 2007 Feb 16;282(7):4453-62. Epub 2006 Dec 15. 10.1074/jbc.M610582200 PubMed 17172467