Rat NCKX2-AS-long-FlagTag (pRc/CMV)
(Plasmid
#75195)
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PurposeMammalian expression of SLC24A2
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 75195 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRc/CMV
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSLC24A2
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Alt nameNCKX2-AS+
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SpeciesR. norvegicus (rat)
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Mutationalternatively spliced exon present
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
FLAG tag is inserted internal to the protein coding region, as annotated in the annotated sequence file.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rat NCKX2-AS-long-FlagTag (pRc/CMV) was a gift from Jonathan Lytton (Addgene plasmid # 75195 ; http://n2t.net/addgene:75195 ; RRID:Addgene_75195) -
For your References section:
Molecular cloning of a novel potassium-dependent sodium-calcium exchanger from rat brain. Tsoi M, Rhee KH, Bungard D, Li XF, Lee SL, Auer RN, Lytton J. J Biol Chem. 1998 Feb 13;273(7):4155-62. PubMed 9461611