Rabbit SERCA2b (pBSII-SK-)
(Plasmid
#75186)
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PurposeCloning vector for ATP2A2
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 75186 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBSII-SK-
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Vector typeBacterial Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATP2A2
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Alt nameSERCA2B
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SpeciesRabbit
- Promoter T7
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rabbit SERCA2b (pBSII-SK-) was a gift from Jonathan Lytton & David MacLennan (Addgene plasmid # 75186 ; http://n2t.net/addgene:75186 ; RRID:Addgene_75186) -
For your References section:
Molecular cloning of the mammalian smooth muscle sarco(endo)plasmic reticulum Ca2+-ATPase. Lytton J, Zarain-Herzberg A, Periasamy M, MacLennan DH. J Biol Chem. 1989 Apr 25;264(12):7059-65. PubMed 2523389