Rat SERCA3 (pMT2)
(Plasmid
#75185)
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PurposeMammalian expression of ATP2A3
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 75185 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMT2
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATP2A3
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Alt nameSERCA3
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SpeciesR. norvegicus (rat)
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Entrez GeneAtp2a3 (a.k.a. SERCA3)
- Promoter adenovirus major late promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pMT2-F
- 3′ sequencing primer pMT2-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rat SERCA3 (pMT2) was a gift from Jonathan Lytton & Gary Shull (Addgene plasmid # 75185 ; http://n2t.net/addgene:75185 ; RRID:Addgene_75185) -
For your References section:
cDNA cloning, functional expression, and mRNA tissue distribution of a third organellar Ca2+ pump. Burk SE, Lytton J, MacLennan DH, Shull GE. J Biol Chem. 1989 Nov 5;264(31):18561-8. PubMed 2553713