Rat SERCA2a (pMT2)
(Plasmid
#75183)
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PurposeMammalian expression of ATP2A2
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 75183 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMT2
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATP2A2
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Alt nameSERCA2A
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SpeciesR. norvegicus (rat)
- Promoter adenovirus major late promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pMT2-F
- 3′ sequencing primer pMT2-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rat SERCA2a (pMT2) was a gift from Jonathan Lytton & Gary Shull (Addgene plasmid # 75183 ; http://n2t.net/addgene:75183 ; RRID:Addgene_75183) -
For your References section:
A novel Ca2+ pump expressed in brain, kidney, and stomach is encoded by an alternative transcript of the slow-twitch muscle sarcoplasmic reticulum Ca-ATPase gene. Identification of cDNAs encoding Ca2+ and other cation-transporting ATPases using an oligonucleotide probe derived from the ATP-binding site. Gunteski-Hamblin AM, Greeb J, Shull GE. J Biol Chem. 1988 Oct 15;263(29):15032-40. PubMed 2844797
