Rat SERCA1a (pMT2)
(Plasmid
#75182)
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PurposeMammalian expression of ATP2A1
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 75182 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMT2
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATP2A1
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Alt nameSERCA1A
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SpeciesR. norvegicus (rat)
- Promoter adenovirus major late promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pMT2-F
- 3′ sequencing primer pMT2-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rat SERCA1a (pMT2) was a gift from Jonathan Lytton (Addgene plasmid # 75182 ; http://n2t.net/addgene:75182 ; RRID:Addgene_75182) -
For your References section:
Molecular cloning and quantification of sarcoplasmic reticulum Ca(2+)-ATPase isoforms in rat muscles. Wu KD, Lytton J. Am J Physiol. 1993 Feb;264(2 Pt 1):C333-41. 10.1152/ajpcell.1993.264.2.C333 PubMed 8447366
