pcDNA3.1-mouse cdk6 D163N
(Plasmid
#75173)
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PurposeMammalian expression of mouse cdk6 with D163N
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 75173 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecyclin dependent kinase 6
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SpeciesM. musculus (mouse)
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MutationD163N
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Entrez GeneCdk6 (a.k.a. AI504062, Crk2)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-mouse cdk6 D163N was a gift from Martine Roussel (Addgene plasmid # 75173 ; http://n2t.net/addgene:75173 ; RRID:Addgene_75173)
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/plasmids/75/75173/75173-map_G-YchQIp7O4S.png.940x940_q85_autocrop.png)