pLC-BFP-FADD
(Plasmid #75166)

• Purpose: LentiCRISPR-BFP with sgRNA targeting human FADD
• Depositing Lab: Beat Bornhauser
• Publication: McComb et al Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 10.1126/scitranslmed.aad2986.

Backbone

• Vector backbone: lentiCRISPRv1
• Total vector size (bp): 11696
• Modifications to backbone: Swap puromycin resistance for TagBFP
• Vector type: Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FADD sgRNA
• Alt name: Fas-Associated Death Domain
• gRNA/shRNA sequence: ACTCCCGCACACGCTCTGTC
• Species: H. sapiens (human)
• GenBank ID: NC_000011.10
• Entrez Gene: FADD (a.k.a. GIG3, IMD90, MORT1)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (Esp3I) (destroyed during cloning)
• 3′ cloning site: BsmBI (Esp3I) (destroyed during cloning)
• 5′ sequencing primer: GACTATCATATGCTTACCGT
• 3′ sequencing primer: AAACGACAGAGCGTGTGCGGGAGTC

Resource Information

• Supplemental Documents:
  • Annotated genbank file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry