pLC-RFP657-CASP8
(Plasmid #75164)

• Purpose: LentiCRISPR-RFP657 with sgRNA targeting human Caspase-8
• Depositing Lab: Beat Bornhauser
• Publication: McComb et al Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 10.1126/scitranslmed.aad2986.

Backbone

• Vector backbone: lentiCRISPRv1
• Total vector size (bp): 11696
• Modifications to backbone: Swap puromycin resistance for tagBFP
• Vector type: Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CASP8 sgRNA
• Alt name: Caspase-8
• gRNA/shRNA sequence: GCCTGGACTACATTCCGCAA
• Species: H. sapiens (human)
• GenBank ID: NG_007497.1
• Entrez Gene: CASP8 (a.k.a. ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (Esp3I) (destroyed during cloning)
• 3′ cloning site: BsmBI (Esp3I) (destroyed during cloning)
• 5′ sequencing primer: GACTATCATATGCTTACCGT
• 3′ sequencing primer: AAACTTGCGGAATGTAGTCCAGGCC

Resource Information

• Supplemental Documents:
  • Annotated genbank file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry