pME-H2B-mCerulean3-P2A-mKOFP2-CAAX
(Plasmid #75154)

• Purpose: Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.
• Depositing Lab: Nicholas Cole
• Publication: Don et al Zebrafish. 2016 Sep 15.

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2900
• Total vector size (bp): 4491
• Vector type: Middle entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H2B-mCerulean3-P2A-mKOFP2-CAAX
• Species: Synthetic
• Insert Size (bp): 1951
• Promoter: n/a

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The H2B-mCerulean3-P2A-mKOFP2-CAAX portion of this plasmid was ordered as a GeneArt™ Genestring™ fragment from ThermoFisher Scientific.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry