His-FGE
(Plasmid #75147)

• Purpose: Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.
• Depositing Lab: Richard Fishel
• Publication: Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.1038/srep16883.

Backbone

• Vector backbone: pET14b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4671
• Total vector size (bp): 5568
• Modifications to backbone: N/A
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FGE
• Alt name: Formylglycine Generating Enzyme
• Alt name: MtFGE
• Alt name: Mycobacterium tuberculosis FGE
• Species: Mycobacterium tuberculosis
• Insert Size (bp): 954
• Mutation: N/A
• GenBank ID: NP_215226
• Promoter: T7
• Tags / Fusion Proteins:
  • Histidine tag (N terminal on insert)
  • Thrombin (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: TAATACGACTCACTATAGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry