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Addgene

pcDNA3-N-HA-NEK7 K64M
(Plasmid #75143)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75143 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 6355
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NIMA (never in mitosis gene a)-related expressed kinase 7
  • Alt name
    Nek7
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    909
  • Mutation
    Lys 64 mutated to Met; kinase inactive and F236L (please see depositor comments below)
  • GenBank ID
    NM_021605
  • Entrez Gene
    Nek7 (a.k.a. 2810460C19Rik, AU020186)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that mutation F236L in NEK7 was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-N-HA-NEK7 K64M was a gift from Bruce Beutler (Addgene plasmid # 75143 ; http://n2t.net/addgene:75143 ; RRID:Addgene_75143)

  • For your References section:

    NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component. Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, Ulevitch RJ, Beutler B. Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 Dec 7. 10.1038/ni.3333 PubMed 26642356
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